Studies on Collagenase Inhibitors. II. Inhibitory Effects of Anthraquinones on Bacterial Collagenase

Inhibitors of Bacterial Collagenase

Corynebacterium rathayil bacterial collagenase

Athanasios YIOTAKIS,* Alain LECOQ,t Anna NICOLAOU,* Jean LABADIEt and Vincent DIVEt§ TDepartment of Organic Chemistry, Laboratory of Organic Chemistry, University of Athens, Panepistimiopolis, Zografou, Athens 15771, Greece, tDepartement d'lng6nierie et d'Etudes des Prot6ines, D.S.V., C.E.A. CE-Saclay, 91191 Gif/Yvette, Cedex, France, and tStation de Recherches sur la Viande, INRA de Theix, 631...

Nature of the inhibitory effect of collagenase on phosphodiesterase activity.

The level of phosphodiesterase (PDE) activity is lower in collagenase-isolated human fat cells than in adipose tissue fragments. The inhibition is not species-specific since collagenase also inhibits PDE in rat adipose tissue and bovine heart. In subcellular fractions from isolated fat cells, the PDE activities were lowest in the plasma membrane-enriched fractions and highest in the cytosolic f...

Immunotargeting of collagenase on thrombus

In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a mo...

Studies on collagen. II. Properties of purified collagenase and its inhibition.

In previous papers from these laboratories (1, 2) methods for the purification of the collagenase of Clostridiurm histolyticum were presented, and a calcium ion requirement for the binding of the enzyme to collagen and for the overall enzymatic action was reported. In the present, communication an additional method of purification, which makes possible the large-scale preparation of the enzyme,...